SUM cell lines and culture requirements. The base medium for all formulations is Ham’s F12 medium.
| Cell Line | Medium | Split Ratio | Trypsin/EDTA
Time |
Extra time in Incubator | Split Frequency | Comments |
| SUM44PE | SFIH | 1:3 | 2 min | no | 7-14 days | Maintain high density |
| SUM52PE | SFIH or 5%IH | 1:6 | 2 min | no | 7-14 days | Cells grow in clumps |
| SUM102PT | SFIHE | 1:6 | 5 min | yes/5 min | 7-14 days | Cells attach strongly |
| SUM1315MO2 | 5%IE | 1:3 | 2 min | no | 7-14 days | Cells detach easily |
| SUM149PT | SFIH or 5%IH | 1:6 | 5 min | yes/10 min | 7 days | Cells attach strongly |
| SUM159PT | 5%IH | 1:10 | 2 min | no | 7 days | Cells resemble fibroblasts |
| SUM185PE | 5%IH | 1:3 | 2 min | no | 7-14 days | Cells grow in clumps |
| SUM190PT | SFIH | 1:3 | 2 min | no | 7-14 days | Cells grow in clumps |
| SUM225CWN | 5%IH | 1:3 | 3 min | no | 7-14 days | Cells grow in patches |
| SUM229PE | 5%IH | 1:3 | 3 min | yes/5 min | 7-14 days | Cells grow in a monolayer |
Abbreviations: SF=Serum Free, 5%=medium containing 5% FBS, H=hydrocortisone, I=Insulin, E=Epidermal Growth Factor, PT=Primary Tumor, PE=Pleural Effusion, CWN=Chest Wall Nodule
Additional Notes:
- SUM44PE cells are density-dependent. Splitting at too low a density will result in no growth of these cells.
- All cell lines are grown in humidified, 37°C incubators with 10% CO2.
- When trypsinizing cells, add trypsin for the indicated time. Then remove trypsin and place “dry” plate in the incubator for the indicated time. Firmly tap the plate on the bench to detach cells before adding medium. Do not forget to add 2% FBS for the first 24 hours to cell lines grown in serum-free medium.
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