The HCC202 human breast cancer cell line was derived from a primary breast ductal carcinoma of an 82 year old Caucasian female. The cell line is an ER-negative, HER2 positive line that exhibits and amplification and overexpression of HER2 (ERBB2). It also is classified as a HER2 amplification subtype based on its expression profile. The STR profile and all genomic information on these cells can be found at Cellosaurus, or at COSMIC, or at DepMap Portal.
Oncogene Signature:
The Oncogene Signature of this cell line is pretty straight forward and is consistent with the lineage characteristics of these cells. The ERBB2 (HER2) oncogene is amplified, overexpressed, and the gene was a hit in both the CRISPR and RNAi functional screens. Thus, HER2 is a clear driving oncogene in this cell line. The cells also have a TP53 point mutation resulting in the almost complete loss of expression of this gene at the message level. Beyond these two clear drivers, there are some other potentially interesting candidate oncogenes that are amplified and overexpressed in these cells, but only CDK12 came close to being a hit in the functional screens.
| Gene | CRISPR score | Demeter score | Log fold change | DNA amp | mutation | occ. In Cosmic |
| ERBB2 | -1.2242987 | -0.528996056 | 5.288860045 | 3.263 | ||
| CDK12 | -0.21976893 | -0.242533461 | 2.317290782 | 2.6486 | ||
| TP53 | -0.169223956 | -0.334040952 | -2.864383907 | -0.5612 | p.T284fs | 25 |
| KDM5C | -0.093546277 | -0.276182306 | 3.354434143 | 3.1631 | ||
| NF1 | -0.078245315 | 0.069159026 | -1.20411873 | -0.5118 | NA | 9 |
| PDGFB | -0.028176101 | 2.535255511 | 1.1381 | |||
| SMC1A | 0.053863415 | -0.079703105 | 3.718372043 | 2.8798 | ||
| HOXB13 | 0.263745493 | 0.053087432 | 4.515093281 | 2.8033 | ||
| SPOP | 0.314442401 | 0.026991263 | 1.57313777 | 1.8541 | ||
| ICOSLG | 1.751783085 | 1.0513 |
HCC202 drug sensitivities. This ERBB2 amplified cell line seems to only be sensitive to ERBB2/HER2 targeted drugs and nothing else. It also should be noted that there are only reported sensitivity data for 14 drugs in the GDSC2 data set, which may explain the paucity of drug sensitivity data for this cell line. There are additional pan-ERBB specific targeted drugs to which the cells are sensitive that appear in Tier 3. Also in Tier 3 are two drugs that have in common BTK as a target to which the cells are sensitive at least in the GDSC1 data set.
| HCC202:T1 | ||||||||
| Drug name | Gene symbol_HGNC | z_score_GDSC1 | z_score_GDSC2 | DNA_amp | lfc | mutation | COSMIC_hit | |
| CI-1033 | ERBB2 | -1.880709 | 0 | 3.263 | 5.288860045 | NULL | 0 | |
| HCC202:T2 | ||||||||
| None | ||||||||
| HCC202:T3 | ||||||||
| Drug name | Gene SymbolHGNC | z_score_GDSC1 | z_score_GDSC2 | DNA_amp | lfc | mutation | achilles_score | demeter |
| QL-XII-47 | BMX | -1.573564 | 0 | -0.1047 | 0 | NULL | 0.109014447 | -0.173033867 |
| LFM-A13 | BTK | -2.136724 | 0 | -1.4904 | 0 | NULL | -0.209648547 | -0.140168442 |
| QL-XII-47 | BTK | -1.573564 | 0 | -1.4904 | 0 | NULL | -0.209648547 | -0.140168442 |
| CI-1033 | EGFR | -1.880709 | 0 | 0.4915 | -0.257281546 | NULL | 0.209937922 | 0.087276944 |
| AST-1306 | EGFR | -2.120082 | 0 | 0.4915 | -0.257281546 | NULL | 0.209937922 | 0.087276944 |
| CI-1033 | ERBB4 | -1.880709 | 0 | -0.513 | 0.646740438 | NULL | 0.229862425 | 0.167131386 |
| AST-1306 | ERBB4 | -2.120082 | 0 | -0.513 | 0.646740438 | NULL | 0.229862425 | 0.167131386 |
