The T47D breast cancer cell line is an ER and PR-positive, HER2 negative breast cancer cell line of the luminal lineage. The cell line was derived from the pleural effusion metastasis of a 54 year old Caucasian female previously diagnosed with infiltrating ductal breast carcinoma. The STR profile along with other genomic data and information pertinent to this cell line can be found here at Cellosaurus, or here at COSMIC or here at DepMap portal.
Oncogene Signature: The Oncogene Signature for this cell line has two functional driving oncogenes; ESR1 (estrogen receptor alpha) and PIK3CA which has one of the most common point mutations seen in breast cancer. The cell line is also TP53 mutant. It is noteworthy that this cell line also highly overexpresses the progesterone receptor (PGR) though this gene was not a hit in the functional seceen
| Gene | CRISPR score | Demeter score | Log fold change | DNA amp | mutation | occ. In Cosmic |
| ESR1 | -0.802285175 | 4.23392322 | -0.3971 | |||
| PIK3CA | -0.922765214 | 0.447875567 | 1.1486 | p.H1047R | 1582 | |
| SOX2 | -0.345256876 | 2.44389832 | 1.1486 | |||
| TP53 | -0.185268306 | 0.486523255 | -0.2882 | p.L194F | 51 | |
| ETV5 | 0.143170867 | -2.589662597 | 1.1486 |
T47D drug sensitivities. This is another ER-positive breast cancer cell line with a PIK3CA mutation. Cells like this often show good sensitivity to both ER-targeted drugs including Tamoxifen, as well as PI3’K targeted drugs. In keeping with the constitutive PI3’K/AKT signaling active in this cell line, the cells also show good sensitivity to several AKT targeted drugs.
| T47D.T1 | |||||||||
| Drug name | Gene symbol_HGNC | z_score_GDSC1 | z_score_GDSC2 | DNA_amp | lfc | mutation | COSMIC_hit | ||
| Fulvestrant | ESR1 | 0 | -1.969162 | -0.3971 | 4.23392322 | NULL | 0 | ||
| GDC0810 | ESR1 | 0 | -1.967374 | -0.3971 | 4.23392322 | NULL | 0 | ||
| Tamoxifen | ESR1 | -3.341002 | -0.882875 | -0.3971 | 4.23392322 | NULL | 0 | ||
| Omipalisib | PIK3CA | -1.847625 | 0 | 1.1486 | 0.447875567 | p.H1047R | 1 | ||
| ZSTK474 | PIK3CA | -1.570202 | 0 | 1.1486 | 0.447875567 | p.H1047R | 1 | ||
| T47D.T2 | |||||||||
| Drug name | Gene symbol_HGNC | z_score_GDSC1 | z_score_GDSC2 | DNA_amp | lfc | mutation | COSMIC_hit | achilles_score | demeter |
| Omipalisib | MTOR | -1.847625 | 0 | -0.3477 | -0.045014109 | p.I177I | 0 | NULL | -0.894033815 |
| T47D.T3 | |||||||||
| Drug name | Gene SymbolHGNC | z_score_GDSC1 | z_score_GDSC2 | DNA_amp | lfc | mutation | COSMIC_hit | achilles_score | demeter |
| Fulvestrant | ESR2 | 0 | -1.969162 | 0.515 | 0.436150056 | NULL | 0 | NULL | 0.078948025 |
| GDC0810 | ESR2 | 0 | -1.967374 | 0.515 | 0.436150056 | NULL | 0 | NULL | 0.078948025 |
| GSK1904529A | IGF1R | 1.14446 | -1.928274 | -0.1514 | 0.801944598 | NULL | 0 | NULL | 0.000327713 |
| GSK1904529A | INSR | 1.14446 | -1.928274 | -0.2488 | -1.306316936 | NULL | 0 | NULL | -0.132739718 |
| OF-1 | BRD1 | 0 | -1.743503 | -0.3308 | -0.111200871 | NULL | 0 | NULL | -0.149497393 |
| OF-1 | BRPF1 | 0 | -1.743503 | 0.1267 | -0.259714689 | NULL | 0 | NULL | -0.056511842 |
| Ipatasertib | AKT3 | 0 | -1.51292 | 0.2183 | -1.587292345 | NULL | 0 | NULL | 0.087644929 |
| Ipatasertib | AKT1 | 0 | -1.51292 | 0.4919 | 0.546594479 | NULL | 0 | NULL | -0.287480497 |
| Ipatasertib | AKT2 | 0 | -1.51292 | -0.573 | -0.209689019 | NULL | 0 | NULL | -0.132871071 |
| MN-64 | TNKS2 | 0 | -1.502366 | 0.6412 | 0.429213798 | NULL | 0 | NULL | -0.33626938 |
| MN-64 | TNKS | 0 | -1.502366 | -0.3503 | -0.114918223 | NULL | 0 | NULL | -0.290443518 |
| AZD6482 | PIK3CB | -2.556663 | -0.663112 | 0.5569 | 0.65677746 | NULL | 0 | NULL | 0.169679506 |
| MK-2206 | AKT1 | -2.080693 | -0.542318 | 0.4919 | 0.546594479 | NULL | 0 | NULL | -0.287480497 |
| MK-2206 | AKT2 | -2.080693 | -0.542318 | -0.573 | -0.209689019 | NULL | 0 | NULL | -0.132871071 |
| AKT inhibitor VIII | AKT3 | -2.582054 | 0 | 0.2183 | -1.587292345 | NULL | 0 | NULL | 0.087644929 |
| GSK690693 | AKT3 | -2.005482 | 0 | 0.2183 | -1.587292345 | NULL | 0 | NULL | 0.087644929 |
| AKT inhibitor VIII | AKT1 | -2.582054 | 0 | 0.4919 | 0.546594479 | NULL | 0 | NULL | -0.287480497 |
| GSK690693 | AKT1 | -2.005482 | 0 | 0.4919 | 0.546594479 | NULL | 0 | NULL | -0.287480497 |
| AKT inhibitor VIII | AKT2 | -2.582054 | 0 | -0.573 | -0.209689019 | NULL | 0 | NULL | -0.132871071 |
| GSK690693 | AKT2 | -2.005482 | 0 | -0.573 | -0.209689019 | NULL | 0 | NULL | -0.132871071 |
| ZSTK474 | PIK3CB | -1.570202 | 0 | 0.5569 | 0.65677746 | NULL | 0 | NULL | 0.169679506 |
| Omipalisib | PIK3CB | -1.847625 | 0 | 0.5569 | 0.65677746 | NULL | 0 | NULL | 0.169679506 |
