The ZR7530 breast cancer cell line is an ER-positive, HER2 positive breast cancer cell line belonging to the HER2 amp molecular sub-type. The cell line was derived from the malignant ascites of a 47 year old female of predominantly (75%) African American heritage. The STR profile along with other genomic data and information pertinent to this cell line can be found here at Cellosaurus, or here at COSMIC, or here at DepMap portal.
Oncogene Signature: In this cell line, ESR1 is highly overexpressed and was found to be esssential in the RNAi based screen, and ERBB2 is amplified and overexpressed and was also found to be a screen hit. One point mutation in the IKZF1 gene was also identified but was not a hit in the screen.
| Gene | CRISPR score | Demeter score | Log fold change | DNA amp | mutation | occ. In Cosmic |
| ESR1 | -0.721298992 | 2.601489172 | -0.3102 | |||
| ERBB2 | -0.594201213 | 5.695009161 | 4.2171 | |||
| IKZF1 | -0.212277965 | -0.013805798 | 0.1504 | p.R328R | 31 | |
| IKZF3 | -0.068886212 | 1.186621672 | 3.7486 | |||
| MSI2 | 0.015891257 | 2.037409709 | 1.3442 | |||
| PPM1D | 0.067761644 | 3.261069593 | 4.0598 |
ZR7530 drug sensitivities. In this cell line, ERBB2 is a functional druggable oncogene, and indeed, the cells are quite sensitive to several specific and pan-ERBB specific targeted drugs. What is most interesting about this cell line however, is the fact that despite the essentiality of ESR1 in these cells, they are actually quite resistant to all ER-targeted drugs. Under standing the mechanism for that resistance in the face of the essentiality of the gene could provide insight into mechanisms of resistance to ER targeted drugs, particularly if it is related to the ERBB status of the cells.
| ZR7530.T1 | |||||||||
| Drug name | Gene symbol_HGNC | z_score_GDSC1 | z_score_GDSC2 | DNA_amp | lfc | mutation | COSMIC_hit | ||
| Afatinib | ERBB2 | -2.084303 | -1.820938 | 4.2171 | 5.695009161 | NULL | 0 | ||
| Lapatinib | ERBB2 | 0 | -1.688538 | 4.2171 | 5.695009161 | NULL | 0 | ||
| ZR7530.T2 | |||||||||
| None | |||||||||
| ZR7530.T3 | |||||||||
| Drug name | Gene SymbolHGNC | z_score_GDSC1 | z_score_GDSC2 | DNA_amp | lfc | mutation | COSMIC_hit | achilles_score | demeter |
| Afatinib | EGFR | -2.084303 | -1.820938 | 0.1504 | -3.425858837 | NULL | 0 | NULL | 0.052392849 |
| Lapatinib | EGFR | 0 | -1.688538 | 0.1504 | -3.425858837 | NULL | 0 | NULL | 0.052392849 |
