The MDA-MB-436 breast cancer cell line is a triple negative cell line that was derived from the pleural effusion metastasis of a 43 year old Caucasian female. This cell line has a BRCA1 mutation, and by molecular profiling has been classified as a Basal B breast cancer cell line. The STR profile along with genomic data and other information pertinent to this cell line can be found here at Cellosaurus, or here at COSMIC, or here at DepMap Portal.
Oncogene Signature: Like most triple negative/basal breast cancer cell lines, this cell line has a pretty basic oncogene signature with only one candidate oncogene scoring as a hit in the RNAi based screen. The cells also have a common TP53 mutation and a mutation in the histone methylase KDMA6, but this was not a screen hit. And, according to Expasy.org, this cell line has a BRCA1; Simple; c.5277+1G>A; ClinVar=VCV000037654; Zygosity=Hemizygous; Note=Splice donor mutation.
| Gene | CRISPR score | Demeter score | Log fold change | DNA amp | mutation | occ. In Cosmic |
| RECQL4 | -0.545446628 | 1.029792712 | 1.330858 | |||
| TP53 | 0.140028931 | -0.323578758 | -0.724387759 | 1.682366 | p.E204fs | 69 |
| RAD52 | 0.173376848 | -0.195588031 | 1.525595682 | 1.154173 | ||
| ERCC5 | -0.229823414 | -0.129842702 | 1.30233113 | 1.243803 | ||
| ETV1 | 0.217039971 | -0.045507528 | 3.596290727 | 1.222762 | ||
| GNA12 | 0.096883093 | -0.043355072 | 1.649761806 | 1.222762 | ||
| KDM6A | 0.370339387 | -0.013061976 | 0.36527691 | 0.283671 | p.L936F | 6 |
| AGO2 | -0.159312213 | -0.00097428 | 1.028279094 | 1.330858 | ||
| PMS2 | -0.21618332 | 1.094616424 | 1.222762 |
| MDA-436.T1 | |||||||||
| None | |||||||||
| MDA-436.T2 | |||||||||
| None | |||||||||
| MDA-436.T3 | |||||||||
| Drug name | Gene SymbolHGNC | z_score_GDSC1 | z_score_GDSC2 | DNA_amp | lfc | mutation | COSMIC_hit | achilles_score | demeter |
| GSK1904529A | IGF1R | -2.617058 | 0.632636 | 0.222329 | 0.922790778 | NULL | 0 | -0.151392298 | -0.03374036 |
| GSK1904529A | INSR | -2.617058 | 0.632636 | -0.592966 | -0.124710129 | NULL | 0 | -0.025841366 | 0.040829729 |
