The HCC1500 breast cancer cell line is an estrogen receptor positive, HER2 negative cell line of the luminal lineage. It was derived from a 32 year old African American woman with ductal breast carcinoma. The STR profile along with other data and references pertinent to this cell line can be found here at Cellosaurus or here at COSMIC, or here at DepMap Portal
The tables below were derived using the data mining tools on the SLKBase and are designed to provide rapid summaries of the oncogene signature of this cell line, and the best druggable targets for these cells.
Oncogene Signature: The Oncogene Signature shows that these cells overexpress ESR1 which is also essential in this cell line. The cells have a CCND1 amplification, and this gene too is essential. Interestingly, these cells slightly overexpress KRAS, which is not mutant in these cells, but the gene still did hit in the screen indicating it’s importance to the proliferative activity of this cell line.
| Gene | CRISPR score | Demeter score | Log fold change | DNA amp | mutation | occ. In Cosmic |
| ESR1 | -1.043697285 | 5.541605976 | 0.376 | |||
| CCND1 | -0.723291063 | 1.837687574 | 2.2584 | |||
| KRAS | -0.55615434 | 1.424442316 | 1.1604 | |||
| BCL2 | -0.461873931 | 5.424356574 | 1.6478 | |||
| ETNK1 | -0.033214331 | 2.153682486 | 1.1604 | |||
| MALT1 | -0.013611167 | 1.898778143 | 1.9138 | |||
| PMAIP1 | 0.015534729 | 3.380845212 | 1.6672 | |||
| TBL1XR1 | 0.025801431 | 1.119210961 | 1.5087 | |||
| INPPL1 | 0.032124927 | 1.747753405 | 1.2156 | |||
| ARHGEF28 | 0.161668712 | 1.188311565 | 1.6187 | |||
| RECQL | 0.176674292 | 1.425066864 | 1.1604 |
HCC1500 Drug sensitivities. The drug sensitivity profile for this cell line is interesting and unexpected given that this is a strongly ER-positive cell line in which the ESR1 gene was a strong hit in the RNAi screen, indicating that this gene is essential for these cells. Despite that essentiality, the cells are not highly sensitive to an ER-targeted drug. By contrast, much like other ER-positive breast cancer cells, the cells overexpress BCL1 and are highly sensitive to a number of different drugs that target BCL2. Pay particular attention to the Z-score for Venetoclax, as this drug is specific for BCL2 and does not target other BCL2 family members. Navitoclax, by contrast targets both BCL2 and BCL2L1, and it’s the BCL2 targeting action of this drug that accounts for it’s low Z-score. Thus, the use of BCL2 targeted drugs in this cell line could significantly sensitize them to other ER-targeted drugs. The other drugs to which the cells are sensitive are pan-specific drugs and the low Z-scores for these drugs were obtained from the GDSC1 screen only.
| HCC1500.T1 | |||||||||
| Drug name | Gene symbol_HGNC | z_score_GDSC1 | z_score_GDSC2 | DNA_amp | lfc | mutation | COSMIC_hit | ||
| Venetoclax | BCL2 | 0 | -3.43015 | 1.6478 | 5.424356574 | NULL | 0 | ||
| ABT737 | BCL2 | 0 | -2.569912 | 1.6478 | 5.424356574 | NULL | 0 | ||
| Navitoclax | BCL2 | -2.220053 | -2.119528 | 1.6478 | 5.424356574 | NULL | 0 | ||
| HCC1500.T2 | |||||||||
| Drug name | Gene symbol_HGNC | z_score_GDSC1 | z_score_GDSC2 | DNA_amp | lfc | mutation | COSMIC_hit | achilles_score | demeter |
| AZD8055 | MTOR | -1.917062 | -0.042388 | -0.671 | -0.67314708 | NULL | 0 | NULL | -1.426706227 |
| HCC1500T3 | |||||||||
| Drug name | Gene SymbolHGNC | z_score_GDSC1 | z_score_GDSC2 | DNA_amp | lfc | mutation | COSMIC_hit | achilles_score | demeter |
| ABT737 | BCL2L10 | 0 | -2.569912 | -0.615 | -0.07038933 | NULL | 0 | NULL | -0.091080511 |
| ABT737 | BCL2A1 | 0 | -2.569912 | -0.5748 | -0.04141993 | NULL | 0 | NULL | -0.068387712 |
| ABT737 | BCL2L1 | 0 | -2.569912 | 0.147 | 0.05361661 | NULL | 0 | NULL | -0.160962775 |
| Navitoclax | BCL2L1 | -2.220053 | -2.119528 | 0.147 | 0.05361661 | NULL | 0 | NULL | -0.160962775 |
| Amuvatinib | FLT3 | -1.972674 | 0 | -0.6433 | 0.770518154 | NULL | 0 | NULL | 0.169902954 |
| Amuvatinib | KIT | -1.972674 | 0 | 0.3623 | -0.06167672 | NULL | 0 | NULL | 0.049255482 |
| Amuvatinib | PDGFRA | -1.972674 | 0 | 0.3623 | -0.05496646 | NULL | 0 | NULL | -0.045576129 |
| Ruxolitinib | JAK1 | -2.40291 | 0.271803 | 0.4204 | 0.287738552 | NULL | 0 | NULL | -0.050407786 |
| Ruxolitinib | JAK2 | -2.40291 | 0.271803 | -0.6589 | 0.511463837 | NULL | 0 | NULL | 0.08318776 |
